Journal: Frontiers in Oncology

Article Title: Modeling extracellular matrix through histo-molecular gradient in NSCLC for clinical decisions

doi: 10.3389/fonc.2022.1042766

Figure Lengend Snippet: Co-analysis of immunofluorescence of chondroitin sulfate (green; C , G , K ) and collagen type I (red; B , F , J ) in three different histological subtypes of non-small cell lung carcinoma (N=120). The stained nuclei are represented in blue (DAPI; A , E , I ). Images D , H , L represent the merge of the same field of these three stains. White arrows indicate positive expression of the markers. Original magnification: 40x. LCC, large cell carcinoma; ADC, lung adenocarcinoma; SqCC: lung squamous cell carcinoma.

Article Snippet: The specimens were incubated overnight at 4°C with antibodies against: E-cadherin (1:100; Boster Biological), β-catenin (1:100; Santa Cruz), heparan sulfate (1:500; Santa Cruz), chondroitin sulfate (1:100, Santa Cruz), anti-human collagen type I (1:700; Rockland Inc.), anti-human collagen type III (1:200; Rockland Inc.), anti-human collagen type IV (1:100; Dako), and anti-human collagen type V (1:1000; Rockland Inc.).

Techniques: Immunofluorescence, Staining, Expressing

Journal: Journal of Pathology and Translational Medicine

Article Title: Malignant potential of neuroendocrine microtumor of the pancreas harboring high-grade transformation: lesson learned from a patient with von Hippel-Lindau syndrome

doi: 10.4132/jptm.2024.02.13

Figure Lengend Snippet: Immunohistochemical results of the pancreatic neuroendocrine microtumor

Article Snippet: Sections were incubated at room temperature for 32 minutes in primary antibodies against ATRX (1:300, Sigma-Aldrich, St. Louis, MO, USA), Bcl-10 (1:400, Santa Cruz Biotechnology, Santa Cruz, CA, USA), CA9 (1:100, Cell Marque, Rocklin, CA, USA), chromogranin (1:200, DakoCytomation, Glostrup, Denmark), cytokeratin 19 (1:100, Cell Marque), DAXX (1:100, Sigma-Aldrich), glucagon (1:200, Cell Marque), insulin (1:1,000, Novocastra, Newcastle upon Tyne, UK), Ki-67 (1:100 DakoCytomation), MUC-1 (1:200, Novocastra), PAX-8 (1:50, Cell Marque), p16 (1:6, Santa Cruz Biotechnology), p53 (1:1,000, DakoCytomation), Rb (1:10,000, QED Bioscience, San Diego, CA, USA), smad4 (1:1,000, Abcam, Cambridge, UK), synaptophysin (1:200, Cell Marque), and vimentin (1:500, Zymed, South San Francisco, CA, USA).

Techniques: Immunohistochemical staining, Over Expression

Journal: Journal of Pathology and Translational Medicine

Article Title: Malignant potential of neuroendocrine microtumor of the pancreas harboring high-grade transformation: lesson learned from a patient with von Hippel-Lindau syndrome

doi: 10.4132/jptm.2024.02.13

Figure Lengend Snippet: Pancreatic neuroendocrine microtumor with high-grade transformation. (A) Double Ki-67 (brown) and synaptophysin (red) immunolabeling shows diffuse synaptophysin positivity in the outer nodule and focal rim positivity in the inner nodule (arrows). Double Ki-67 (brown) and synaptophysin (red). (B) Diffuse chromogranin positivity in the outer nodule and focal positivity in the inner nodule (arrows). (C) Diffuse but weak p53 labeling is only observed in the inner nodule (arrows). (D) Carbonic anhydrase 9 is diffusely positive in the outer nodule but is negative in the inner nodule (arrows). (E) Intact smad4 labeling in the inner nodule (arrows) is diminished in the outer nodule. (F) Vimentin expression is present in the outer nodule only and is absent in the inner nodule (arrows).

Article Snippet: Sections were incubated at room temperature for 32 minutes in primary antibodies against ATRX (1:300, Sigma-Aldrich, St. Louis, MO, USA), Bcl-10 (1:400, Santa Cruz Biotechnology, Santa Cruz, CA, USA), CA9 (1:100, Cell Marque, Rocklin, CA, USA), chromogranin (1:200, DakoCytomation, Glostrup, Denmark), cytokeratin 19 (1:100, Cell Marque), DAXX (1:100, Sigma-Aldrich), glucagon (1:200, Cell Marque), insulin (1:1,000, Novocastra, Newcastle upon Tyne, UK), Ki-67 (1:100 DakoCytomation), MUC-1 (1:200, Novocastra), PAX-8 (1:50, Cell Marque), p16 (1:6, Santa Cruz Biotechnology), p53 (1:1,000, DakoCytomation), Rb (1:10,000, QED Bioscience, San Diego, CA, USA), smad4 (1:1,000, Abcam, Cambridge, UK), synaptophysin (1:200, Cell Marque), and vimentin (1:500, Zymed, South San Francisco, CA, USA).

Techniques: Transformation Assay, Immunolabeling, Labeling, Expressing

Journal: Cancer Medicine

Article Title: Improved outcomes of localized diffuse large B‐cell lymphoma at the Waldeyer ring in comparison to the sinonasal area in the rituximab era

doi: 10.1002/cam4.6851

Figure Lengend Snippet: Immunohistochemical staining results of diffuse large B‐cell lymphoma involving the Waldeyer ring, sinonasal area, or lymph nodes without extranodal involvement.

Article Snippet: Antihuman primary antibodies against MYC (clone Y69; ab32072; Abcam, Cambridge, UK), BCL2 (clone SP66; ab236221; Abcam), p53 (clone DO7; 453 M‐96; Cell Marque, Rocklin, California, United States), and CD5 (clone SP19; 205R‐16; Cell Marque) were used to detect the corresponding proteins.

Techniques: Immunohistochemistry, Staining, Expressing

Journal: Cancer Medicine

Article Title: Improved outcomes of localized diffuse large B‐cell lymphoma at the Waldeyer ring in comparison to the sinonasal area in the rituximab era

doi: 10.1002/cam4.6851

Figure Lengend Snippet: Immunohistochemical staining for MYC, BCL2, and p53 overexpression in diffuse large B‐cell lymphoma involving the Waldeyer ring (WR‐DLBCL), sinonasal area (SN‐DLBCL), or lymph nodes without extranodal involvement (LN‐DLBCL).

Article Snippet: Antihuman primary antibodies against MYC (clone Y69; ab32072; Abcam, Cambridge, UK), BCL2 (clone SP66; ab236221; Abcam), p53 (clone DO7; 453 M‐96; Cell Marque, Rocklin, California, United States), and CD5 (clone SP19; 205R‐16; Cell Marque) were used to detect the corresponding proteins.

Techniques: Immunohistochemistry, Staining, Over Expression

Journal: PPAR Research

Article Title: PPAR- γ Ligand Inhibits Nasopharyngeal Carcinoma Cell Proliferation and Metastasis by Regulating E2F2

doi: 10.1155/2019/8679271

Figure Lengend Snippet: Expressions of E2F2 and PPAR- γ in clinical samples of undifferentiated NPC and NPG and associations with clinicopathological characteristics of undifferentiated NPC samples.

Article Snippet: After three washes with phosphate-buffered saline (PBS), the sections were incubated in a blocking solution containing bovine serum albumin (BSA) for 20 min, followed by exposure to primary antibodies against E2F2 (YT1443, Immunoway, rabbit polyclonal, 1:200 dilution) and PPAR- γ (Novusbio, rabbit polyclonal, 1:100 dilution) at 4°C overnight.

Techniques: Expressing

Journal: PPAR Research

Article Title: PPAR- γ Ligand Inhibits Nasopharyngeal Carcinoma Cell Proliferation and Metastasis by Regulating E2F2

doi: 10.1155/2019/8679271

Figure Lengend Snippet: E2F2 and PPAR-γ protein expression in nonkeratinizing nasopharyngeal carcinoma (NPC) and nasopharyngitis (NPG) tissues . Immunohistochemistry was performed to detect E2F2 and PPAR- γ protein expression in nonkeratinizing NPC tissues (a, b) and NPG tissues (c, d). Scale bar, 50 μ m (e, f). The immunohistochemistry data were analyzed semiquantitatively to determine the E2F2 and PPAR- γ expression levels in nonkeratinizing NPC and NPG tissues. Data are shown as means ± standard deviations. ∗∗ P <0.01.

Article Snippet: After three washes with phosphate-buffered saline (PBS), the sections were incubated in a blocking solution containing bovine serum albumin (BSA) for 20 min, followed by exposure to primary antibodies against E2F2 (YT1443, Immunoway, rabbit polyclonal, 1:200 dilution) and PPAR- γ (Novusbio, rabbit polyclonal, 1:100 dilution) at 4°C overnight.

Techniques: Expressing, Immunohistochemistry

Journal: PPAR Research

Article Title: PPAR- γ Ligand Inhibits Nasopharyngeal Carcinoma Cell Proliferation and Metastasis by Regulating E2F2

doi: 10.1155/2019/8679271

Figure Lengend Snippet: E2F2 and PPAR-γ protein expression in nonkeratinizing nasopharyngeal carcinoma (NPC) and nasopharyngitis (NPG) tissue lysates . (a, b) RT-PCR and Western blotting were performed to detect the expression of E2F2, PPAR- γ , and GAPDH in lysates of nonkeratinizing NPC and NPG tissues. (c, d) Quantitative analyses of the E2F2 and PPAR- γ expression levels in nonkeratinizing NPC and NPG tissues. Data are expressed as means ± standard deviations. ∗ P <0.05; ∗∗ P <0.01.

Article Snippet: After three washes with phosphate-buffered saline (PBS), the sections were incubated in a blocking solution containing bovine serum albumin (BSA) for 20 min, followed by exposure to primary antibodies against E2F2 (YT1443, Immunoway, rabbit polyclonal, 1:200 dilution) and PPAR- γ (Novusbio, rabbit polyclonal, 1:100 dilution) at 4°C overnight.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: PPAR Research

Article Title: PPAR- γ Ligand Inhibits Nasopharyngeal Carcinoma Cell Proliferation and Metastasis by Regulating E2F2

doi: 10.1155/2019/8679271

Figure Lengend Snippet: E2F2 and PPAR-γ expression in CNE1 and CNE2 nasopharyngeal carcinoma cells after treatment with PPAR-γ ligand . (a, b) Western blotting was performed to detect the expression of E2F2, PPAR- γ , and GAPDH in CNE1 and CNE2 cells after treatment with dose-dependent PPAR- γ agonist Rog. (c, d) Western blotting was performed to detect the expression of E2F2, PPAR- γ , and GAPDH in CNE1 and CNE2 cells after treatment with PPAR- γ agonist (rosiglitazone, Rog) and PPAR- γ antagonist (GW9662).

Article Snippet: After three washes with phosphate-buffered saline (PBS), the sections were incubated in a blocking solution containing bovine serum albumin (BSA) for 20 min, followed by exposure to primary antibodies against E2F2 (YT1443, Immunoway, rabbit polyclonal, 1:200 dilution) and PPAR- γ (Novusbio, rabbit polyclonal, 1:100 dilution) at 4°C overnight.

Techniques: Expressing, Western Blot